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Molagri

Sr. Research Associate, Yeast Expression and VLP Production

Toronto, ON, Canada

$85,000 to $105,000 USD / year · Full-time

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Job details

Pay: $85,000 to $105,000 USD / year
Job type: Full-time
Posting language: English
Posted: Oct 6, 2026

Original job description

We are seeking a hands-on research associate to produce engineered VLPs in yeast and build production, purification, QC, and stability workflows.

You will help build Molagri’s yeast VLP production workflows. This role will focus on expressing engineered VLPs in yeast, improving yield and recovery, purifying particles, running routine QC, and screening storage conditions that preserve particle integrity and payload quality.

You will work closely with our virology and protein biochemistry teams to produce purified VLP material for characterization, insect-cell assays, and insect bioassays.

You will:

Engineer strains of yeast to accommodate efficient cell growth and VLP yields at manufacturing scale.
Work closely with viral and biochemistry teams to genetically modify yeast strains to test new VLP variants in a timely manner.
Transform yeast with VLP expression constructs and screen clones for their expression, yield, and particle quality.
Produce VLPs in shake flasks and small-scale bioreactors.
Test and optimize production conditions such as growth media, induction strategy, temperature, harvest time, and other process variables.
Run harvest, lysis or clarification, purification, buffer-exchange, and concentration workflows.
Run routine QC assays for expression, particle assembly, purity, aggregation, RNA packaging, and RNA integrity.
Support stability screens across pH, salt, excipients, temperature, freeze-thaw, and storage conditions.
Troubleshoot low-yield expression, poor recovery, particle instability, aggregation, RNA loss, and inconsistent QC results.
Maintain SOPs, batch records, assay records, and sample inventories.

What You Bring

BSc or MS in biochemistry, molecular biology, microbiology, biochemical engineering, biotechnology, or a related field.
Hands-on experience expressing recombinant proteins, VLPs, or other biological products in yeast or microbial systems.
Experience with yeast transformation, clone screening, shake-flask expression, and small-scale fermentation or bioreactor runs.
Experience with protein and/or particle purification methods involved in the preparation of samples and downstream protocols such as chromatography, filtration.
Working knowledge of routine protein, RNA, or particle QC methods such as SDS-PAGE, Western blot, ELISA, qPCR, HPLC/SEC, DLS, or plate-based activity assays.
Precise/meticulous bench technique and the ability to reproducibly run many small production and QC experiments.
Strong recordkeeping skills, including clear batch recording, assay notes, sample tracking, and SOP updates.
Ability to troubleshoot production workflows and communicate results clearly to scientists across virology, protein biochemistry, and insect biology.
Excitement to work at the bench in a small team where production workflows are still being built.
Self-starter with a drive to grow with their role alongside the company’s trajectory.

What We Value

Molagri is a small team building a novel bioinsecticide platform. We value scientists who design rigorous experiments, think creatively when the obvious approach fails, communicate clearly, and treat their teammates with kindness and respect. The right person for this role will be hands-on, hard-working, intellectually honest, and excited to help build a platform from first principles.

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More about this job

Responsibilities

Engineer and screen yeast strains for VLP production, optimize expression and small-scale production conditions, and purify particles for downstream research. Run routine quality-control and stability assays, troubleshoot production issues, and maintain accurate SOPs, batch records, assay records, and sample inventories.

Requirements

A BSc or MS in a relevant life-science or engineering field and hands-on experience expressing recombinant proteins, VLPs, or other biological products in yeast or microbial systems are required. Candidates should have experience with yeast transformation, clone screening, small-scale fermentation, purification, routine protein/RNA/particle QC, meticulous recordkeeping, and cross-functional communication.

Skills

Yeast Transformation
Yeast Strain Engineering
VLP Expression
Clone Screening
Shake-Flask Expression
Small-Scale Fermentation
Bioreactor Operation
Protein and Particle Purification
Chromatography
Filtration
Protein and RNA Quality Control
SDS-PAGE
Western Blot
ELISA
qPCR
Stability Screening

Benefits

Equity

Visa sponsorship

Not detected in the job text

Keywords

Yeast Expression
Virus-Like Particles
VLP Production
Yeast Strain Engineering
Yeast Transformation
Clone Screening
Shake-Flask Production
Small-Scale Bioreactors
Fermentation
Growth Media Optimization
Induction Strategy
Harvesting
Cell Lysis
Clarification
Particle Purification
Buffer Exchange
Concentration
SDS-PAGE
Western Blot
ELISA
qPCR
HPLC/SEC
Dynamic Light Scattering
RNA Packaging
RNA Integrity
Stability Screening
Batch Records
Standard Operating Procedures
Bioinsecticide

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